摘要
本文报道了利用ＰＣＲ技术检测了３４例丙型肝炎病毒Ｅ２／ＮＳ１基因的ｃＤＮＡ并与５’非编码区基因的ｃＤＮＡ检测技术进行了比较，应用ｐＵＣ１８质粒对此扩增片段进行了克隆与序列分析，结果表明克隆片段ＨＣＶ－Ｓ１为我国主要流行的ＨＣＶ基围亚型－ＩＩ型，其与ＨＣＶ－ＩＩ型的核苷酸与氨基酸的同源性均在９０％以上，序列分析表明该片段富含脯氨酸和胱氨酸，可能具有复杂的空间构型，且与４个糖基化位点一样保持稳定，另外，在ＨＣＶ－Ｓ１片段的３’端有一３６个核苷酸的区域。
The 3’ end fragment of E2/N S1
gene was amplified with polymerase chain reaction(PCR) techni-que and
then cloned it to pUC18 vector and sequenced.The results showed the
inserted fragment(HCV-S1) had high homology with HCV genotype
II,whose nucleotide and amil1oacid homology were morethen 90%. Itwas
similar to major epidemic strain in China. The diversity the
fragment has beencharacterized .We have also detected 34 hecatitis C
cases by RT-pCR using E2 /NS1 gene primers(E2/NS1 PCR)and compared
it using 5 ’noncoding region primers(5’NCR PCR)。
出处
《北京医科大学学报》
CSCD
1994年第3期163-167,共5页
Journal of Peking University(Health Sciences)
关键词
丙型肝炎病毒
丙型肝炎
基因克隆
Hepatitis
C virus(HCV)
Hepatitis C
Gene cloning
Gene sequencing
Polymerasechain reaction(PCR)
