摘要
以北京鸭红细胞提取的ＨＭＧｓ（ＨＭＧ＿１，ＨＭＧ＿（２ａ），ＨＭＧ＿（２ｂ），ＨＭＧ＿（１４），ＨＭＧ＿（１７））免疫Ｂａｌｂ／ｃ小鼠，取其脏压细胞与Ｓｐ２／０（小鼠骨髓瘤细胞）融合，经选择培养，阳性筛选，克隆化以及冻存复苏，获得了三株稳定分泌抗ＨＭＧ＿（１４）的杂交瘤细胞株（１Ｃ＿（１２），１Ｏ＿３和２Ｆ＿２），经ＷｅｓｔｅｒｎＢｌｏｔ鉴定，与其它几种ＨＭＧ无反应，抗体为ＩｇＧ＿１亚型。腹水ＨＭＧ＿（１４）单克隆抗体经ＳｅｐｈａｄａｘＣＬ－４Ｂ亲和柱得到了纯化。
Spleen cells from Balb/c mice immunied with duckred blood cell HMG were fused with murine myeloma cell line Sp2/0. By selective culture,screening and subcloning three hybridomas 1C_(12),1O_3and2F_2 were obtained which secreted mono-clonal antibody(MoAb)against HMG stably. 1C_(12),1O_3 and 2F_2 MoHb had strong reaction with HMG,but not react with oth-er HMG and H_(12). These three moAbs are IgG_1 pattern.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
1994年第6期740-746,共7页
Acta Scientiarum Naturalium Universitatis Pekinensis
