摘要
本研究采用体外细胞毒方法（ＭＴＴ）检测了重组人肿瘤坏死因子（ｒＨＴＮＦ）和（或）阿霉素（ＡＤＭ）对肝癌细胞株ＳＭＭＣ─７７２１及Ｂｅｌ─７４０２的细胞毒效应。结果表明：ｒＨＴＮＦ、ＡＤＭ对两肝癌细胞株的毒性有明显差异，ｒＨＴＮＦ与ＡＤＭ联合应用的体外细胞毒试验显示良好的协同增强效应。在此基础上，选用ＳＭＭＣ─７７２１细胞株进行棵鼠移植瘤试验，观察ｒＨＴＮＦ与ＡＤＭ联合应用的体内抗瘤效应。在治疗６个周期后，空白对照的肿瘤大小为：２．３８７±０．９７ｃｍ３，ＡＤＭ与ｒＨＴＮＦ联合应用组仅为０．４３±０．１８９ｃｍ３，两者相比Ｐ＼０．００１。联合用药对肿瘤生长的抑制率为８２．０７％，明显高于单用ＡＤＭ组（３８．８３％）和单用ｒＨＴＮＦ组（２７．３％），联合用药的协同增强抑制率为１５．９４％。由此再次证明：ｒＨＴＮＦ与ＡＤＭ联合抗瘤作用并非简单的作用叠加，体外及裸鼠体内试验均显示协同增强的抗瘤效应。本实验为肝癌的治疗提供了实验依据。
In order to investigate the value of tumor necrosis factor(TNF)combined with adriamycin against human hepatocarcinoma, we have used MTT method to examine the in vitro cytotoxic activity of rhTNF or and ADM against two human hepatocarcinoma cell lines: SMC-7721 and BEL-7402. The results showed that the cytoto-xic effects of rhTNF against these two cell lines were different. We found that rhTNF singnificantly enhanced the cytotoxic activity of ADM. Based on experiments in vitro, we have examined also in vivo tests. using SMMC-7721 tumor-bearing nude mice. The anti-tumor effect was evident. The addition of rhTNF to ADM resulted in a singnificant re-duction in tumor volume at six cycle treatment compared to untreated animals. The ave-rage volumes of the tumors were 2.387±0.97cm3 and 0.43±0.189cm3 respectively (p<0.01). The tumor growth inhibition rate was 82.07 % for the combination group as compared to that of 38.83% for ADM alone group and 27.3% for the rhTNF alone group. These result showed that rhTNF plus ADM had a synergistic enhancement of antineoplastic activity in vivo. In conclusion, from the study of vivo and vitro, we believe that some hepaticcarcinoma patients will be benefit from receiving treatment with rhTNF plus chemotherapy.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1994年第6期500-502,513,共4页
Chinese Journal of Cancer
关键词
肝癌细胞系
肿瘤坏死因子
阿霉素
裸鼠
体外细胞毒
Hepatocarcinoma cell line Tumor Necrosis Factor Adriamycin MTT assay Nude mouse
