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ABC-ELISA定量检测血小板相关IgG 被引量:4

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摘要 本文介绍应用国产BAS试剂建立检测PAIgG的ABC-ELISA方法。实验因素探讨表明。ABC-ELISA灵敏度较Pc Ab-ELISA次8倍、较McAb高2倍,因而用血量较少;同时,本法还具有重复性好、简便、稳定等优点。应用于临床对ITP的诊断及疗效观察很有意义。
出处 《上海医学检验杂志》 1989年第3期145-148,共4页 Shanghai Journal of Medical Laboratory Sciences
关键词 ABC ELISA 血小板 IGG
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参考文献1

  • 1Peng Xiaolian,Wang Bianming,Shen Di,Wei Wenning. Clinical value of detecting platelet—associated IgG in patients with idiopathic thrombocytopenic purpura[J] 1982,Acta Academiae Medicinae Wuhan(3):170~175

同被引文献22

  • 1张之南.血液病诊断及疗效标准(第二版)[M].北京:科学出版社,1999.271-272.
  • 2Hou M, Andersson P O, Stockelderg D et al. Plasmathromgbopoietin levels in thrombocytopenic states: impli-cation for a regulatory role of bone marrow megakary-ocytes. BrJ Haematol, 1998, 101: 420
  • 3Sakane Y, Wada H, Kazuyo H et al. Increased throm-bopoietin levels in idiopathic thrombocytopenic purpurapatients with a poor response to steroid therapy. BloodCoagul Fibrinolysis, 1998, 9:315
  • 4Porceliji L, Folman C C, Bossers B et al. The diagnosticvalue of thrombopoietin level meansurements in thrombo-cytopenia. Thromb Haemost, 1998, 79: 1101
  • 5Hiyoyoama K, Wada H, Shimura M et al. Increased serumlevels of thrombopoietin in patients with thromboticthrombocytopenic purpura, idiopathic thrombocytopenicpurpura, or disseminated intravascular coagulation. BloodCoagul Fibrinolysis, 1997, 8: 345
  • 6Kuter D J, Rosenberg R D. The reciprocal relationshipof thrombopoietin to changes in the platelet mass duringbusulfan-induced thrombocytopenia in the rabbit. Blood,1995, 85:2720
  • 7Hirayama Y, Sakamaki S, Matsunaga T et al. Concentra-tions of thrombopoietin in bone marrow in normal sub-jects and in patients with idiopathic thrombocythpenicpurpura, aplastic anemia, and essential thrombocythemiacorrelate with its mRNA exprission of bone marrow stro-mal cells. Blood, 1998, 92:46
  • 8Kamura T, Handa H, Hamasaki N. Characterization ofthe human thrombopoietin gene promoter, a possible roleof an its transcription factor, E4TF1/GABP. J BiolChem, 1997, 272:11361
  • 9Chang M, Qian J X, Lee S M et al. Tissue uptake of cir-culating thrombopoietin is increased in immune-mediatedcompared with irradiated thrombocy topenic mice.Blood, 1999, 93:2515
  • 10Murray L J, Luens K M, Estrada M F et al. Throm-bopoietin mobilizes CD34+ cell subsets into peripheralblood and expands multilineage progenitors in bone mar-row of cancer patients with normal hematopoiesis. ExpHematol, 1998, 26:207

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