摘要
钳夹大鼠肾蒂30分钟,再开放血流60分钟,肾皮质Na-K-ATP酶活性(2.99±1.66)比对照肾(3.68±0.96)下降18.6％(P<0.05)。分别给予缺血鼠FDP4g/kg和丹参18g/kg,其试验肾酶活性(5.03±2.25、3.26±0.59)分别和对照肾(3.87±1.06,2.90±0.59)相比,均无显著差异(P>0.05);分别给予正常鼠FDP4g/kg和丹参18g/kg,前者试验肾酶活性(4.14±0.74)比对照肾(3.24±0.73)升高(P<0.05),后者无区别(分别为3.16±1.43和3.70±0.98,P>0.05)。本实验提示FDP和丹参能对抗缺血所致的肾皮质Na-K-ATP酶活性的下降。
After 30 min of pedicle clamping and 60 min of reflow (after right nephrectomy), the left renal cortical Na-K-ATPase activity (2.99±1.66μM Pi/mg protein/h) was found significantly lower than that of the control kidneys (3.68±0.96, P<0.05). A dose of 4g/kg body weight of fructose 1,6- diphosphate (FDP) was administered for 60 min starting immediately after reflow in ischemic group and for 90 min starting immediately after right nephrectomy in untreated group. The values were 5.03±2.25 and 4.14±0.74μM Pi/mg protein/h respectively in the two groups. They were found higher than those of the control kidneys (3.87±1.06, P>0.05 and 3.24±0.73, P<0.05 respectively). A dose of 18g/kg body weight of DANSEN (Salvia miltiorrhiza Bunze, a Chinese herb) was administered in the same manner as described above. The values were 3.26±0.59 and 3.61±1.43μM Pi/mg protein/h respectively. In the ischemic and untreated groups, there were no significant change as compared with the control kidneys (2.90±0.59, P>0.05 and 3.07±0.98μM Pi/mg protein/h, P>0.05 respectively). It is concluded as follows: 1. 30 min of pedicle clamping could result in decline of renal cortical Na-K-ATPase activity. 2. FDP and DANSEN could prevent the decline of renal cortical Na-K-ATPase activity induced by ischemia. 3. FDP could raise renal cortical Na-K-ATPase activity in normal rats but DANSEN had no such effect.
出处
《上海医学》
CAS
CSCD
北大核心
1989年第4期187-189,共3页
Shanghai Medical Journal
