摘要
应用蔗糖密度梯度离心及乙醇分级沉淀法从分泌抗人甲状腺球蛋白单克隆抗体的杂交瘤组织中提取mRNA。微量注射mRNA于蛙卵母细胞。72h后以~3H—Leu参入值测定蛋白质合成增加,并以免疫沉淀方法证明了mRNA注射蛙卵产物具有抗甲状腺球蛋白抗体的免疫活性。
Messenger RNA was prepared from hybridoma secreting monoclonal antihuman thyroglobulin antibodies. Micro-injection of the mRNA into frog oocyteS resulted in increasing of protein synthesis detected by incorporation of 3H-Leu. the immunological activity of antibodies against thyroglobulin was proved in the products of oocytes after mRNA injection by immuno- precipitation.
出处
《白求恩医科大学学报》
CSCD
1989年第1期27-29,共3页
Journal of Norman Bethune University of Medical Science
关键词
MRNA
杂交瘤
蛙卵
mRNA Translation Micro-injection
