摘要
本文报告了用长波紫外线(UVA)照射结合补骨脂衍生物灭活血浆中指示细小病毒 M13Mpl8的初步结果。当血浆中加入8-MOPSOOμg/ml,UVA 强度为11.5mW/cm^3时,30到120分钟照射可以杀灭10^(5～9)传染剂量/ml 的病毒。照射防护剂可以减轻 UVA 对蛋白质的损伤以提高照射后凝血因子的回收率。2mmol/L 谷胱苷肽或合用2mmol/L 甘露醇可明显提高凝血因子Ⅶ(FW)的回收率。
The preliminary results of inactivation of model parvovirus M13Mp 18 in plasma by long-waveUVA irradiation combined with psoralen derivatives were described.At 8-MOP concetration of300μg/ml plasma and UVA intensity of 11.5mW/cm^2,the UVA irradiation for 30～120 min couldresult in virus inactivation of 10^(5～9) infectious dose/ml.Quenchers were used to reduce the damage ofUVA to proteins in order to improve the clotting factor recovery after irradiation.2mmol/Lglutathione,or 2mmol/L glutathione with 2 mmol/L mannitol could significantly improve the Frecovery after irradiation.
出处
《中国输血杂志》
CAS
CSCD
1993年第4期178-181,共4页
Chinese Journal of Blood Transfusion
关键词
血浆
病毒
灭活
紫外线
光敏化合物
:UVA
Psoralen derivatives
Parvovirus
Plasma virus inactivation
