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人工诱导旱稻结瘤固氮的探索研究 被引量:1

Studies on Inducing Nodulation and Nitrogen Fixation of Upland Rice
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摘要 采用终浓度为1ppm的2,4-D处理旱稻苗,无论是否接种固氮微生物,均能使其根部形成瘤状物。结瘤率、单株瘤数及瘤大小与接种菌无关,但不同旱稻品种的结瘤率有差异。乙炔还原测定表明,接种具离体固氮酶活的固氮微生物的稻苗,在某些情况下,能显示极微弱的固氮酶活,但与该稻苗是否用2,4-D处理尚无必然联系。稻瘤的含菌结瘤率约占总瘤数的33%~40%。初步鉴定稻瘤分离菌系原接种菌。 It was found that the nodulation of upland rice roots could be induced by lppm final concentration of 2, 4-D, whether N2-fixing microorganisms inoculated or not. The nodulation rate, number and the diameter of para-nodules seemed to have no relevance to the inocula. There are differenles of nodulation rates with different varieties of. the rice. Gas chromatograph showed that some of the rice seedlings infected by the inocula with Nase activities in vitra had exhibited very weak Nase activities in some cases. However, no relation to treatment of 2, 4-D and nase activity could be found in this experiment. The nodulation rate containing the bacteria was about 33%~40% of total para-nodules of upland rice. The bacteria isolated from the para-nodules was'preliminarily identified to be original inoculum.
作者 丁武
出处 《中国农学通报》 CSCD 1993年第1期14-17,共4页 Chinese Agricultural Science Bulletin
基金 总理基金
关键词 旱稻 2 4-D 结瘤 固氮酶活 Upland rice 2,4-D Nodulation Nase activity
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同被引文献23

  • 1张丽梅,方萍,朱日清.禾本科植物联合固氮研究及其应用现状展望[J].应用生态学报,2004,15(9):1650-1654. 被引量:36
  • 2海伟力,王耀东,尤崇杓,周慧玲.水稻根际联合固氮细菌的研究[J].微生物学报,1993,33(2):79-85. 被引量:11
  • 3尤崇杓,林敏,方宣钧,许崇任,张尚通,冯九.联合固氮遗传工程菌的间释放与风险评价[J].农业生物技术学报,1995,3(1):34-41. 被引量:2
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  • 5王子芳 郭军.联合固氮细菌在生态系统氮素循环中的意义[J].微生物学通报,1987,14(4):171-174.
  • 6D(o)bereine J, DayJ M.Associative symbioses and nitrogen-fixing sites[A].In:Newton W E,Nyman C J eds. International Symposium on Nitrogen fixation[C].Pullman Proceedings Washington State University, 1975.518- 538.
  • 7Cavalcanteva V A, D[o]bereiner J. Anew acid-tolerant nitrogen-fixing bacterium associated[J]. Plana and Soil, 1988,108:23 - 25.
  • 8Urquiaga S,Cruz K H S,Boddey R M. Contribution of nitrogen fixation to sugar cane:Nitrogen-15 and nitrogen-balance estimates[J]. Soil Sci Soc Amer Proc,1992,56:105 - 114.
  • 9丘元盛 周淑萍 莫小真.水稻根际固氮细菌的研究[J].科学通报,1980,19(25):1008-1008.
  • 10Dobereiner J.Non-symbiotic nitrogen fixing bacterin in tropieal soils[J]. Proc Natl Acad Sci, 1975, 72:2383-2373.

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