摘要
以NT—P—BSA为抗原,用ELISA测定969例健康人及974例麻风病人血清中的抗PGL—Ⅰ抗体(IgM)。1.健康女性的OD及阳性率显著高于男性,应分别按其OD确定阳性值;2.以常态曲线为基础,按OD±2SD为阳性阈值的计算法用到抗PGL—Ⅰ抗体是不合宜的;3.麻风流行率降到一定程度后,这种测试不能反映些微差异,只有在发病率高的疫点才可用此法监察流行动态;4.不活动的MB及PB病人,其OD及阳性率仍明显高于健康人,其意义有待研究;5.在不同时期和地点测试应统一方法和试剂,还要用质控血清校正结果。
Sera from 969 healthy subjects and 974 leprosy patients were tested for anti -PGI, - I antibody (APGL-I) by the ELISA technique with NT-P-BSA. The results showed that 1) the mean OD and positivity of healthy female were significantly higher than those of healthy-males and similar tendency occured between sex groups of leprosy patients, suggesting that the female positive criteria of OD must be separate from that in the male, 2)The levels of PGL-I were skew in distribution in all groups except the MB patient group, suggesting it unsuited to calculating the cut-off point of OD according co mean OD+2SD based on normal distribution ,3) When the leprosy prevalence was reduced to a certain extent, this detectioncould not reflect slight difference, but might reflect epidemiological dynamics in some epidemic points with relatively higher incidence, 4) There were still sighnificantly higher mean OD and positivity in inactive MB and PB patients than in healthy subjects. It remains to be shown what is clinically and epidemio logically significant, and if they could serve as an applicable parameter for relapse, 5) In order to guarantee reliability of detection, it must be emphasized that standardized methods and materi als, especially the control pool sera,are neessary for correct results, at different times and in different laboratories.
