摘要
用传统方法(TM,包括生化和培养特性)和现代方法(MM,包括Mc Ab—ELISA及巢式引物基因扩增试验—NPGAA)对57株从临床标本中分离出的AFB培养物进行鉴定。从人麻风瘤分离出的AFB培养物的代表株(M.A1、7、13、21及22),用TM鉴定为不同于已知标准分技菌的新种,而用McAb—ELISA和NPGAA则鉴定为不属于麻风菌。从疑似结节病病人皮损中分离的培养物代表株(M.S17、1、2、2R、7及23),对M.S17仅用TM即鉴定为瘰疬分枝菌;其它株用TM和MeAD—ELISA鉴定,表明其不同于麻风菌,而与结核菌密切相关,用NPGAA,则鉴定为结核菌,作者认为虽然MM对检测的鉴定分枝菌要比TM敏感和特异得多,但TM和MM都非常有用,尤其是两者结合能相互补充。更加完美使如何选择这些方法的单用或联用,要依研究目的及实际需要而定。
This article reports the results of identifying 57 AFB cultures isolated from clinical specimens by using traditional methods (TM, including mainly biochemical and cultural properties) and modern ELISA with monoclonal antibody (McAb -ELISA) and nested primer gene amplification assay (NPGAA). The representatives (M. A1,A7,A15. A21 and A22) of AFB cultures isoltated from human lepromas were identified as new species different from known standard mycobacteria by TM, while not identical to M. leprae by McAb-ELISA and NPGAA; As for the representatives (M. S17,S1,S2,S22,S7,S25),M. S17 was found to be identical to M. scrofulaceum by using TM only,others (M. S1 ,S2,S22,S7,S23) were identified as mycobacteria close to M. tuberculosis and different from M. leprae with TM and McAb-ELIASA, as M. tuberculosis with NPGAA. From these studies, it is considered that the TM and modern methods (MM) are all very useful in identifying mycobacteria either alone or integrately, although MM was much more sensitive and specific to detection and identifcation of mycobacteria than TM, especially integration of TM and MM could not only complement each other, but also become more complete. The way of selecting and using these methods alone or integrately depends on the purposes and practical needs.
