摘要
用LR White低温包埋法及胶体金标记免疫电镜细胞化学技术对保护性单克隆抗体M26-32识别的体外培养的恶性疟原虫FCC1/HN株红内期145/102 kDa抗原进行超微结构定位研究。发现金颗粒主要定位在该株原虫红内期环状体、滋养体、裂殖体及裂殖子的细胞质中;一些金颗粒则定位于原虫表面的复合膜或散布在感染红细胞的细胞质中。表明145/102kDa抗原是恶性疟原虫FCC1/HN株红内期各无性发育阶段的共同细胞质抗原,其一部分可途经原虫表面的复合膜而被转运到感染红细胞的细胞质。
The ultrastructural localization of the 145/102 kDa antigens recognized by the possible protective monoclonal antibody (MeAb) M26-32 in erythrocytic stages of Plasmodium falci-parum, FCC1/HN, in vitro, was investigated by immuno-electron microscopy with LR White resin embedding and colloidal gold probe cytochemistry techniques. The results showed that the gold particles were mainly localized within the cytoplasm of ring forms, trophozoites,schizonts and merozoites of the Plasmodium. Some gold particles were found to locate on the pellicular complex of the plasmodium surface or in the cytoplasm of the infected erythrocytes. The results indicated that 145/102 kDa antigens were the common cytoplasmic antigens of asexual blood stages of Plasmodium falciparum, FCC1/HN, while a portion of the antigens could be transported to the cytoplasm of the infected erythrocytes via the pellicular complex of the plasmodium surface (Figs. 1-4).
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第2期102-104,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
联合国开发计划署/世界银行/世界卫生组织热带病研究培训特别规划资助课题(880153)
关键词
疟原虫
抗原
胶体金标记
超微结构
Plasmodium falciparum,antigen,monoclonal antibody,colloidal gold labeling, ultrastructural localization
