摘要
采用正交试验设计,观察环境温度、光照周期、脾脏切除和雌激素水平对大鼠体内约氏疟原虫红细胞外期生长发育的影响。结果显示:脾脏在清除子孢子方面有重要作用,切脾组的红外期原虫密度显著高于对照组;雌激素水平的升高则明显抑制红外期的发育。低温环境对红外期的密度虽无明显影响,但使之发育变缓,不同步更为显著。光照周期的长短似乎对红外期的生长发育无明显影响。两种因素交互作用时,脾脏切除和雌激素水平升高的影响相互抵消,其他两两因素的作用,经方差分析,均未见显著性差异。
Using orthogonal design, the effect of environmental temperature, photoperiod , splenec-tomy and oestrogen levels upon the development of exoerythrocytic forms (EEF) of P. yoelii was observed. The results indicated that a significant number of viable sporozoites were cleared in the spleen, since the EEF density was much higher in the livers of splenectomized rats (1. 73/mm3)than in sham-operated counterparts(0. 55/mm3). The effect of high level oestrogen on EEF density was also evident since there was a significant difference between experimental(0. 86) and control rats(1. 42). Low environmental temperature caused the development of EEF stunted and asynchronous,but no significant effect on the density of EEF in this group was found. The density and average diameter (AD) of EEF between rats illuminated 8 and 16 hours per day were identical. The interaction between splenectomy and elevated oestrogen level offset each other, whereas the interactions between other two factors showed no difference by analysis of variance.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1993年第2期98-101,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
约氏疟原虫
红细胞外期
发育
Plasmodium yoelii, exoerythrocytic stage, development, orthogonal test
