摘要
用套式多聚酶链反应(NPCR)和普通PCR法检测丙型肝炎病毒核酸比较。NPCR溴化乙锭染色法结果与^(32)P标记探针核实结果完全一致,无漏检,也无假阳性,可不用^(32)P标记探针核实。NPCR法的敏感性为普通PCR溴化乙锭染色法的2倍,为普通PCR加探针法的1.71倍。
The sensitivity and specificity of nested polymerase chain reaction (NPCR)for detection of HCV RNA was compared with general polymerase chain reaction (PCR), and confirmed by specific probe of HCV RNA labeled with 32p. The results of NPCR observed by ethioum bromide stainning was identical with 32p probe, i.e. there was no false negative and no false positive. The sensitivity of NPCR for detection of HCV RNA was 2 times of PCR method, amd 1.71 times of PCR and 32p probe method.
