摘要
采用磷酸铅沉淀的电镜酶化学技术,研究了莼菜(Bresenia shreberi Geml.)茎表皮腺毛细胞中酸性磷酸酶(ACPase)及碱性磷酸酶(ALPase)的分布及其在腺毛发育过程中的活性变化。ACPase活性在头细胞进入粘液分泌期时才开始出现,主要分布于头细胞的内质网、角质膜、液泡膜和柄细胞的核膜、胞间连丝、壁内突附近质膜以及基细胞壁内突和附近质膜上。ALPase活性在头细胞形成初期首先出现在基细胞的线粒体内嵴上。在头细胞粘液分泌期该酶主要分布在头细胞内质网、角质膜、部分线粒体内嵴和柄细胞的核膜、内质网、部分高尔基体、胞间连丝、壁内突及附近质膜上,基细胞中的线粒体反应最强烈。表明ACPase和ALPase在腺毛发育过程中起着促进细胞分化改组和细胞间物质转运的作用;证实莼菜腺毛头细胞合成粘液的原料是由基细胞及周围薄壁组织提供的。
The ultrastructural distribution of acid phosphatase (ACPase) alkaline phosphatase (ALPase) and the changes of their activities in the laminar hair cells of Bresema skreberi Geml.were studied by means of lead phosphat preciptation method during their development.The ACPase activity became detactable at the stage of mucilage secretion of the head cell,which was located at endoplasmic reticulum (Er),cuticle (Cu) and tonoplast (Tp) of the head cell,and nuclear membrane (NM) ,plasmodesmata (P1) and wall ingrowths (WI) of the stalk cell,and WI and plasmolemma of basal cell.ALPase activity showed its first appearance at the mitochondria (Mi) of the basal cell at the stage of head cell formation.At the stage of head cell secretion,ALPase activity was located at Er,Cu,Mi of head cell,and NM,Go,Er,WI and P1 of stalk cell.ALPase activity at Mi of basal cell was most intensive.The results indicated that ACPase and ALPase possessed the function of promoting cell differentiation and material transfer in the process of hair cell development and that the material for synthesizing mucilage in head cell was provided by the basal cells and the parenchyma cells nearby.
基金
国家自然科学基金资助项目
关键词
酸性磷酸酶
碱性磷酸酶
腺毛
莼菜
Acid phosphatase
Alkaline phosphatase
Cytochemical electron microscopy
Glandular hair
Bresenia shreberi
