摘要
分析用多聚酶链反应（ＰＣＲ）方法检测的５４６例次结果的临床意义。其中儿科病例约占三分之二。结果：①结核杆菌１９１例，４１．４％阳性；②巨细胞病毒１８８例，３５．６％阳性；③弓形体６５例，１５．４％阳性；④乙肝病毒４８例，３９．５％阳性；⑤ＥＢ病毒２９例，３４．５％阳性；⑥单纯疱疹病毒Ⅰ型７例，未发现阳性；⑦肺炎支原体４例，未发现阳性；⑧β地贫反向点杂交４例，５０％阳性；⑨单纯疱疹病毒Ⅱ型１例，未发现阳性；⑩淋球菌１例，未发现阳性。结核ＰＣＲ阳性与临床符合率为８０．６％；巨细胞病毒在脑损伤患者阳性率为５６．４％，明显高于非脑损伤患者（２０．４％）；差异有非常显著意义（Ｐ＜０．０１）；弓形体在脑损伤患者阳性率为１７．１％，明显高于非脑损伤患者（３０例中未检出）；乙肝ＨＢｓＡｇ阳性的１１例患者中有１０例ＰＣＲ阳性，两者符合率高达９０．９％；２例β地贫患儿其中１例为双重杂合子（ＣＤ１７，Ａ→Ｔ；ＩＶＳ６５４，Ｃ→Ｔ），另１例为杂合子（ＩＶＳ６５４，Ｃ→Ｔ）。
The clinical significance of detection of polymerase chain reaction (PCR) was analyzed in 546 cases,in which the child cases were account for two third. Results were shown as follow.1) the positive rate of tuberculosis PCR was 41.4%in 191 cases;2) cytomegalovirus was 35.6% in 188 cases;3)toxoplasma was 15.4%in 65 cases;4) hepatitis Bis 39.5%in 48 cases;5) EB virus is 34.5% in 29cases; 6) herpes simplex virus type I is none in 7 cases;7)mycoplasma pneumonia was none in 4 cases; 8) reverse dot blot for beta-thalasemia was 50%in 4 cases; 9) herpes simplex virus type II was none in 1 case;10) gonococcus was none in 1 case. 80.6% cases with positive tuberculosis PCR conformed with clinical diagnosis.The positive rate of cytomegalovirus was 56.4% in brain damage cases, which was higher apparently than in the non-brain damage cases (20.4%), the difference shows statistically significant (P<0.01). The positive rate of toxoplasma is 17.1%in brain damage cases and also higher than in non-brain damage cases(none out of 30 cases).There were 10 cases of positive hepatitis B PCR in 11 cases with positive hepatitis B sAg, the conformable rate got up to 90.0%. Of two cases of thalasemia, there was one double heterozygote at codon 17,A→T and IVS 654, C→T; another was heterozygote at IVS 654, C→T.
出处
《中国优生与遗传杂志》
1995年第2期1-4,共4页
Chinese Journal of Birth Health & Heredity
