摘要
背景:精神疾病患者是易发生自杀的高危人群,目前有关自杀的神经生物学和遗传学研究主要集中于5-HT系统。有研究发现5-HT2A受体基因的T102C多态性与抑郁症患者的自杀有关联。目的:探讨5-羟色胺2A(5-HT2A)受体基因A-1438G多态性与精神疾病患者的自杀未遂之间的关系。设计:以患者为研究对象,以健康体检者为对照组的观察对比研究。单位:一所市级医院的精神科和一所大学医院的神经科。对象:本研究所有病例的标本收集于2002-3/2002-9在扬州市五台山医院精神科完成,基因型分析在2002-10/2002-12于徐州医学院神经生物学实验室完成。病例纳入标准:符合《中国精神障碍分类与诊断标准》第3版(CCMD-3)和《美国精神障碍诊断与统计手册》第4版(DSM-Ⅳ)“精神分裂症”或“情感障碍”诊断标准。病例排除标准:有自伤的意愿或行为,但并不真正想死的准自杀者。根据有无自杀行为或意图/意念将116例患者分为2组,即自杀未遂组52例(男35例,女17例),平均年龄(44±13)岁;无自杀组64例(男44例,女20例),平均年龄(48±15)岁。正常对照组63例(男33例,女30例),平均年龄(55±17)岁。方法:所有入组者按常规氯仿-饱和酚白细胞提取法提取DNA,用限制性片段长度多态性方法分析5-HT2A受体基因的多态性。
BACKGROUND:Psychiatric patients are the high risk group of people who are liable to commit suicide.Recently,research on neurobiology and genetics of suicide mainly concentrates in the study of 5 hydroxytryptamine(5 HT) system.It is found that T102C polymorphism of 5 HT2A receptor gene is associated with suicide in depressive patients.OBJECTIVE:To investigate the correlation of A 1438G polymorphism of 5 HT2A receptor gene with attempted suicide in psychiatric patients. DESIGN:An observational comparative study of taking the patients as subjects and healthy physical examinee as the controls.SETTINGS:A psychiatric department in a municipal hospital and a neurological department in a university hospital.PARTICIPANTS:All the samples in the research were collected from the Department of Psychiatry of Yangzhou Wutaishan Hospital from March to September 2002.Analysis of genotype was completed in the Neurobiological Laboratory of Xuzhou Medical Institute from October to December 2002.Inclusive criterion:patients met the diagnostic criteria of schizophrenia or affective disorder in the third edition of the Chinese Classification and diagnostic criteria of Mental Disorders(CCMD 3) and Diagnostic and Statistical Manual of Mental Disorders Fourth Edition(DSM IV).Exclusive criterion: parasuicider with idea or behavior of hurting oneself,but without intention to die.According to whether there was suicidal behavior or intention,116 patients were divided into 2 groups:attempted suicide group[n=52,35 males and 17 females,with an average age of (44±13) years old] and no suicide group [n=64, 44 males and 20 females, with an average age of (48±15) years old].Other 63 cases were taken as the normal controls[33 males and 30 females, with an average age of (55±17) years old].INTERVENTIONS:DNA of all the participants was extracted with the routine method of chloroform saturatedphenol leukocyte pheresis.The polymorphism of 5 HT2A receptor gene was analyzed with the restriction fragment length polymorphism.MAIN OUTCOME MEASURES:①Comparison of allele frequencies for A1438G polymorphism in the 5 HT2A receptor gene and genotype distribution in the 3 groups;②Comparison of allele frequency for A 1438G polymorphism in the 5 HT2A receptor gene and genotype distribution in schizophrenia and affective disorder in attempted suicide group.RESULTS:The G allele frequency of A 1438G polymorphism in the attempted suicide group(0.52) was significantly higher than that in the normal control group(0.39)(χ2=3.91,P< 0.05).There were significant differences in the distribution of the 3 genotypes of AA, AG and GG between the attempted suicide group(0.23,0.50, 0.27) and normal control group(0.32, 0.59, 0.09)(χ2=6.12,P< 0.05).CONCLUSION:Attempted suicide of psychiatric patient is associated with A 1438G polymorphism of 5 HT2A receptor gene.G allele may be a risk factor for the attempted suicide of psychiatric patient.
出处
《中国临床康复》
CSCD
北大核心
2005年第4期238-240,共3页
Chinese Journal of Clinical Rehabilitation
