摘要
微量DNA提取法是目前以PCR法鉴定转基因番茄植株T1代单株幼苗的一种简便、快速、有效的方法.该方法需约1 h即可完成植物DNA的提取,样品用量仅为30-100mg,产量可达30-80μg/g,粗提DNA(溶于10μlTE缓冲液)不必经过纯化,用TE缓冲液稀释5-10倍即可直接用于PCR分析.
At Prensent,trace DNA extraction is a convenient,quick and effective way of identifying individual T1 seeding of transgenic tomato plants with PCR.The to- tal time for the DNA extraction of the way is approximately an hour.Samples needed were only 30—100mg and DNA yield could reach 30—80μg/g.The rude isolation DNA (dissolved in 10μl of TE buffer) do not need to be purified and could be directly used to PCR analysis after being diluted to 5～10 times.
基金
中国人民解放军总后勤部卫生部青年基金(01Q128)
浙江飞虹集团企业风险基金(2000-1)
