摘要
目的 :通过检测AFPmRNA在肝细胞癌 (hepatocellularcarcinoma ,HCC)患者肝组织和外周血中的基因表达 ,探讨AFPmRNA相对定量值作为HCC微转移指标的可能性 ,建立HCCAFPmRNA相对定量分析法。方法 :建立敏感的巢式逆转录聚合酶链反应体系 (Nested RT PCR) ,对肝组织和外周血中的AFPmRNA进行相对定量值分析。结果 :在 49例HCC及其癌旁和远癌组织、12例非肝肿瘤组织和 8例正常肝组织中AFPmRNA相对定量值阳性分别为 3 5例 ( 71 4% )、2 0例 ( 4 0 8% )、3例 ( 6 1% )、0例、0例 ;3 5例肝癌患者中有2 3例术前外周血AFPmRNA相对定量值为阳性 ( 65 7% ) ,明显高于肝硬化、急性肝炎、慢性肝炎患者和正常人外周血 ;HCC患者外周血术后 72hAFPmRNA相对定量值的阳性率 2 2 9% ( 8/3 5 ) ,与术前比较差异有统计学意义 ,P <0 0 1。结论 :肝组织和外周血中AFPmRNA相对定量分析可提高HCC阳性诊断率 ,并可作为HCC微转移的早期检测指标。
OBJECTIVE:To investigate the expression of alpha-fetoprotein(AFP) mRNA in hepatic tissues and peripheral blood of hepatocellular carcinoma (HCC) and probe the possibility of relative quantitative assay in AFP mRNA as a marker of micrometastasis in HCC, and establish the method of relative quantitative assay of AFP mRNA in HCC.METHODS:A sensitive nested-RT-PCR system was set up to make relative quantitative values of AFP mRNA of hepatic tissues and peripheral blood.RESULTS:The positive rate of relative quantitative values of AFP mRNA in 49 cases of hepatoma tissues, paracancerous tissues, distal cancerous tissues, 12 cases of nonhepatic tumor tissues and 8 cases of normal hepatic tissues were 71.4%(35/49),40.8%(20/49),6.1%(3/49),0 and 0, respectively; 23 of the 35 HCC patients (65.7%) were positive for relative quantitative values of AFP mRNA in the peripheral blood before operation and significantly higher than those in cirrhotic, acute hepatitis, chronic hepatitis patients and normal persons. The positive rate of relative quantitative values of AFP mRNA in the peripheral blood from HCC patients 72 h after operation was 22.9%(8/35), in which there were statistically significant differences compared with that of pre-operation,P<0.01.CONCLUSIONS:Relative quantitative assay of AFP mRNA in hepatic tissues and peripheral blood can improve the positive rate in diagnosis of HCC, and could be an earlier detective indicator of micrometastasis in HCC. The changes before treatment are after treatment are of important reference values for instructing clinical therapy and evaluating the effects of treatment of HCC.
出处
《肿瘤防治杂志》
2004年第12期1250-1253,共4页
China Journal of Cancer Prevention and Treatment
基金
山东省卫生厅资助项目 ( 2 0 0 1CAIDADA5 )
关键词
癌
肝细胞/病理学
肝肿瘤/病理学RNA
信使/分析
聚合酶链反应
相对定量分析
carcinoma, hepatocellular/pathology
liver neoplasms/pathology
RNA, messenger/analysis
polymerase chain reaction
relative quantitative assay
