摘要
Background Human embryonic stem (HES) cell derived from human blastocyst can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent It has exciting potential in human developmental biology, drug discovery, and transplantation medicine But there are insufficieot HES cell lines for further study Methods Three oocyte donors were studied, and 3 in vitro fertilization (IVF) cycles were carried out to get blastocysts for the establishment of HES cell line Isolated from blastocysts immunosurgically, inner cell mass (ICM) was cultured and propagated on mouse embryonic fibroblasts (MEFs) Once established, morphology, cell surface markers, karyotype and differentiating ability of the cell line were thoroughly analyzedResults Four ICMs from 7 blastocysts were cultured on MEFs After culture, one cell line (cHES1) was established and met the criteria for defining human pluripotent stem cells including a series of markers used to identify pluripotent stem cells, morphological similarity to primate embryonic stem cells and HES reported else where. Normal and stable karyotype maintained over 60 passages, and demonstrated ability to differentiate into a wide variety of cell types Conclusions HES cell lines can be established from gamete donors at a relatively highly efficient rate The establishment will exert a widespread impact on biomedical research
Background Human embryonic stem (HES) cell derived from human blastocyst can be propagated indefinitely in the primitive undifferentiated state while remaining pluripotent It has exciting potential in human developmental biology, drug discovery, and transplantation medicine But there are insufficieot HES cell lines for further study Methods Three oocyte donors were studied, and 3 in vitro fertilization (IVF) cycles were carried out to get blastocysts for the establishment of HES cell line Isolated from blastocysts immunosurgically, inner cell mass (ICM) was cultured and propagated on mouse embryonic fibroblasts (MEFs) Once established, morphology, cell surface markers, karyotype and differentiating ability of the cell line were thoroughly analyzedResults Four ICMs from 7 blastocysts were cultured on MEFs After culture, one cell line (cHES1) was established and met the criteria for defining human pluripotent stem cells including a series of markers used to identify pluripotent stem cells, morphological similarity to primate embryonic stem cells and HES reported else where. Normal and stable karyotype maintained over 60 passages, and demonstrated ability to differentiate into a wide variety of cell types Conclusions HES cell lines can be established from gamete donors at a relatively highly efficient rate The establishment will exert a widespread impact on biomedical research
基金
ThisstudywassupportedbyagrantfromtheNaturalScience FoundationofGuangdongProvince
China(No 083017)
