摘要
应用PCR SSCP的方法对大白猪、长白猪和杜洛克猪的Kappa阿片受体 (Kappaopioidreceptor,简称KOR)基因进行单核苷酸多态性检测和分析 ,研究Kappa阿片受体基因作为候选基因 ,影响母猪行为规癖性状的可能性。根据Kappa阿片受体基因外显子的部分序列设计 3对引物 ,发现F1/R1引物对扩增的片段有多态性。对两种纯合子片段克隆并测序表明 ,mRNA第 10 5处有一C→T的单碱基突变 ,为沉默突变。统计结果发现 ,3种基因型 (AA、AB、BB)在各品种中的分布不一致 ,χ2 独立性检验差异极显著 (P <0 0 1)。将 3种基因型同行为规癖性状进行统计分析 ,结果表明BB基因型与其他两种基因型相比表现较高的静止站立行为 ,同AA和AB型比较差异极显著 (P<0 0 1) ,其他 4种行为性状基因型间差异不显著。因此 。
The possibility of the Kappa opioid receptor(KOR) gene as the candidate gene affecting the stereotyped behavior traits in sows was discussed in the current study.Based on the exon partial sequence of sow KOR gene,three pairs of primers were designed to analyze single nucleotide polymorphisms to develop PCR-SSCP method in Landrace,Yorkshire and Duroc breeds.One polymorphism,found in PCR product amplified with F1/R1,was caused by a single nucleotide mutation of C to T at the position of mRNA 105 compared with the sequence published in GenBank,which was a silence mutation.The results of χ~2 test showed that the frequencies of genotypes in different breeds were different significantly (P<0.01).The least square analysis between three genotypes and the stereotyped behavioral traits showed that individuals with BB genotype had significantly higher (P<0.01) still standing behaviors than those with AA and AB genotype,but no difference were fund in other behavioral traits.According to the above results,we may conctude that SNP of KOR gene has effect on the still standing behavioral trait of sows。
基金
国家自然科学基金资助 (项目编号 :3 9970 5 3 3 )~~
