摘要
目的:研究EBV相关胃癌(EBV associated gastric carcinomas,EBVaGC)和EBV阴性胃癌(EBV negative gastric carcinomas,EBVnGC)组织中c-Met基因的扩增及表达,探讨c-Met基因扩增和表达与EBV感染的相关性以及二者在胃癌发生发展中的作用. 方法:应用半定量多聚酶链反应(PCR)检测EBVaGC 13 例和EBVnGC 45例组织中c-Met基因的扩增,免疫组化抗原热修复技术检测c-Met蛋白表达. 结果:EBVaGC和EBVnGC组织中均检测到c-Met基因扩增,EBVaGC组织与EBVnGC组织间c-Met基因的扩增水平无显著性差异.EBVaGC癌组织c-Met基因过度扩增率为53.8%(7/13),EBVnGC癌组织c-Met基因过度扩增率为44.4%(20/45),两组之间无差异. EBVaGC和EBVnGC癌组织中c-Met蛋白阳性率分别为76.9%(10/13)和64.4%(29/45),两组之间无显著性差并.EBVaGC和EBVnGC癌组织中c-Met蛋白过表达率分别为69.2%(9/13)和37.8%(17/45),统计学分析表明EBVaGC癌组织中c-Met蛋白的过表达高于EBVnGC 组,两组之间有差异(X2=4.0 345,P=0.0 446). EBVaGC和EBVnGC癌组织中c-Met基因的表达和扩增与肿瘤大小、肿瘤部位、淋巴结转移、分化程度以及临床分期等均无相关性. 结论:胃癌组织中均存在c-Met基因的扩增,EBVaGC 与EBVnGC组织中c-Met基因扩增水平和c-Met蛋白阳性率无明显差异,但EBVaGC癌组织中c-Met蛋白的过表达率高于EBVnGC组.
AIM: To detect the amplification and expression of c-Met in Epstein-Barr virus (EBV)-associated gastric carcinoma (EBVaGC) and EBV-negative gastric carcinoma (EBVnGC), and to investigate the relationshi pof the amplification and expression of c-Met with EBV infection in the process of gastric carcinogenesis. METHODS: The amplification of c-Met gene and the expression of c-Met protein in the tissues of EBVaGC (n = 13) and EBVnGC (n = 45) were detected by semi-quantitative polymerase chain reaction (PCR) and immunohistochemi-cal methods, respectively. RESULTS: The amplification of c-Met was observed in both EBVaGC and EBVnGC. The overamplification rates were 53.8% (7/13) in EBVaGC and 44.4% (20/45) in EBVnGC. There was no significant difference between the two types of caners. c-Met protein was positive in 76.9% (10/13) EBVaGC, and 64.4% (29/45) EBVnGC tissues. The difference between them was not significant either. The overexpression rate of c-Met protein in EBVaGC was significantly higher than that in EBVnGC (69.2% vs 37.8%, P=0.0 446). No significant correlation was observed between the amplification and expression of c-Met with the tumor size, location, lymph node metastasis, differentiation degree or clinical staging. CONCLUSION: c-Met is amplified in both EBVaGC and EBVnGC tissues. The amplification level of c-Met gene and the positive rate of c-Met protein are not significantly different between EBVaGC and EBVnGC. However, the overexpression rate of c-Met protein in EBVaGC is higher than that in EBVnGC.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第3期312-316,共5页
World Chinese Journal of Digestology
