摘要
目的 用不同来源的藻酸盐建立藻酸盐免疫性肺炎动物模型 ,以研究藻酸盐对肺组织的免疫原性及致病作用。方法 本实验以生理盐水作对照 ,分别用海藻藻酸盐、细菌藻酸盐免疫家兔。测定三组家兔的体温外周白细胞计数及分类计数 ;对支气管肺泡灌洗液 (BALF)进行细菌计数 ;观察气管及肺组织的病理变化。结果 家兔经两种藻酸盐免疫后外周血白细胞数及BALF细菌培养菌落数与对照组比较差异无统计学意义。镜下观察可见细支气管旁淋巴细胞浸润、淋巴小结形成及细支气管管腔狭窄等病理改变。结论 用海藻藻酸盐和细菌藻酸盐都能建立稳定的免疫性肺炎动物模型 ,藻酸盐可导致肺组织发生病理性炎症反应。
Objective To reproduce a rabbit model of alginate immune pneumonia, and to study the pathogenic role of alginate on lung tissue. Methods Rabbits of group A, being challenged by saline, served as the control group. Rabbits of group B and C were immunized with seaweed alginate and bacterial alginate respectively. Body temperature,peripheral blood cells and the serum level of circulating immune complex (CIC) were measured after the challenge. Bronchoalveolar lavage fluid (BALF) specimens were incubated and bacterial colonies were counted. The cell number and differential count of BALF were made. The pathological changes of the lung tissue were observed under microscopy. Results In both seaweed alginate and bacterial alginate groups, the peripheral blood cell count and the number of bacterial colonies in BALF were similar to that of the saline group. However, the serum CIC levels and the cell count of BALF were increased significantly after the challenge. Under light microscopy, lymphocyte infiltration and lymphoid follicle formation around the bronchiolus were found in all alginate immunized groups, and there was also narrowing of bronchioli. Conclusion Both seaweed alginate and bacterial alginate could be used to establish the model of alginate immune pneumonia in rabbits. Alginate can cause inflammatory reaction and pathological changes in the rabbit lung, manifesting mainly peribronchilar lymphocytic infiltration and lymphoid follicle formation around the bronchioli.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2005年第2期135-138,共4页
Medical Journal of Chinese People's Liberation Army
关键词
藻酸盐
免疫
肺炎
疾病模型
动物
alginates
immunity
pneumonia
disease model, animal
