摘要
目的 :研究染料木黄酮对去势大鼠骨骼矿化的影响。方法 :雌性Wistar大鼠 4 7只随机分为假手术组 ,去势对照组、去势 +雌激素组 (己烯雌酚 2 0 μg .kgbw-1.d-1)、去势 +染料木黄酮组 (剂量分别为 2 5、5 0、10 0mg .kgbw-1.d-1)。饲养三个月后处死 ,测定骨密度、骨矿化相关参数、骨钙、磷、锌、镁、锰、血清甲状旁腺激素、降钙素和雌激素含量。结果 :大鼠去势后 ,股骨骨密度降低 ,平均类骨质宽度增大 ,骨矿化延迟时间和类骨质成熟时间延长 ,骨中钙、磷、锌、镁和血清雌激素含量降低 ,与假手术组相比均有显著性差异 (P <0 .0 5 ) ;补充染料木黄酮后 ,股骨骨密度有改善的趋势 ,平均类骨质宽度变窄 ,骨矿化延迟时间和类骨质成熟时间缩短 ,骨中钙、磷、镁含量升高。结论 :染料木黄酮通过促进类骨质矿化 ,减少骨中钙、磷、镁丢失 ,预防骨质疏松的发生。
Aim: To investigate the effects of genistein on bone mineralization in ovariectomized rats. Methods: Forty-seven Wistar rats were randomly allocated into six groups: sham-operated(sham), ovariectomized(ovx), ovariectomized supplied with diethyl stilbestrol(E, 20 μg.kg bw -1 .d -1 ) or genistetin(25, 50, 100 mg.kg bw -1 .d -1 ). After the rats had been fed for three months, analysis of the bone mineral density, parameters related to mineralization, bone content of Ca, P, Mg, Mn and Zn and serum concentration of parathyroid calcitonin and estrogen was performed. Results: Bone mineral density, bone Ca, P, Zn and Mg content and serum estrogen concentration in ovariectomized rats were significantly decreased, but mean osteoid width increased, mineralization lag time and osteoid maturation period prolonged compared with sham animals. After three months supplementation to ovariectomized rats, bone Ca, P and Mg content increased, mean osteoid width decreased, mineralization lag time and osteoid maturation period shortened compared with ovariectomized animals. Conclusion: Genistein promotes bone mineralization by increasing bone Ca, P, Mg and adjusting serum calcitonin to prevent osteoporosis.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2005年第1期86-89,共4页
Chinese Journal of Applied Physiology
