摘要
本文研究了三甲基奶油黄(3—Me—DAB)诱发的大鼠肝癌组织内r—谷氨酰转肽酶(γ—GT)免疫组织化学定位,以及血清及肝癌组织中γ—GT活性和谷胱甘肽硫转移酶(GST)活性水平之间的关系。8只实验性肝癌大鼠血清及肝癌组织中γ—GT水平均明显高于9只对照组,血清及肝癌组织中GST水平明显低于9只对照组(P—0.01)。免疫组化组织中γ—GT活性测定相符合。
Activity of glutathione S-transferase (GST) and r-glutamyl transpeptidase (γ-GT) in rat serum and liver during 3-methyl-4-dimethylaminoazobenzene(3-Me-DAB) induced hepatocarcinogenesis was investigated and assayed with immunohistochemical localization of γ-GT. During 3-Me-DAB hepatocarcinogenesis in rats γ-GT activities of serum and liver cancer tissue were increased obviously. It is considered to be one of the best markers for the prene-oplastic lesion of experimental hepatoma.Immunohistochemical staining, using a spcific antiserum against rat kidney γ-GT, demonstrated that positive staining for γ-GT was observed in experimental hepatoma tissues, in the biliary canaliculi-like spaces, on the cell membrane a!nd sometimes in the cytoplasm of malignant cells. The results showed that immunohistochemical localization of γ-GT in tissues correlated well with γ-GT activity of the tissues.The levels of GST of serum and tissue were reduced in primary hepatoma caused by feeding 3-Me-DAB. The GST level was strikingly decreased at the late stage of hepatocarcinogenesis in 3-Me-DAB induced primary hepatomas. Furthermore, it may be important to study the specific antibodies against the GST for the purpose of diagnosis and treatment of primary hepatoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1989年第1期17-20,共4页
Chinese Journal of Cancer
