摘要
目的 :检测细胞标志蛋白及P5 3在子宫内膜癌细胞的表达 ,探讨它们参与不同亚型子宫内膜癌发病的可能机制 ,分析其与肿瘤生物学行为的关系。方法 :采用免疫组织化学法检测 131例子宫内膜癌患者不同亚型、不同临床分期及不同分化程度的子宫内膜细胞中CK7,Vim及P5 3的表达。结果 :CK7及Vim的表达分别与P5 3的表达存在正相关性。内膜样腺癌细胞中CK7表达高于其他类型 ,而Vim及P5 3的表达降低。Vim的表达与临床分期呈正相关 ;Vim及P5 3的表达阳性率随细胞分化级别上升而增加 ,具有相关性。结论 :CK7、Vim、P5 3的编码基因可能通过转录水平的相互调控机制参与不同亚型子宫内膜癌发生及发展过程 ,CK7,Vim及P5 3可作为判断肿瘤的预后及选择治疗方案有用的参考指标。
Objective: Analyzing the protein expression of biomarkers CK7, Vim, and P53 to investigate their possible pathogenic roles in the development of variant subtypes of endometrial carcinoma. Methods:Biomarkers CK7, Vim, and P53 were immunohistochemistry-stained among 131 endometrial carcinoma specimens including 93 endometroid, 8 adenoacanthoma, and 32 rare subtypes of adenosquamas carcinoma, clean cell carcinoma, and papillary carcinoma, which had been confirmed clinically and pathologically, and studied statistically with Fisher test and Cochran-Mantel-Haenszel (CMH) Test. Results: Positive correlation was demonstrated among CK7, Vim, and P53 expression levels. The CK7 protein expression is increased, while the Vim and P53 are decreased in the subtype of endometrioid carcinoma. The clinical staging of endometriroid carcinoma is positively correlated with the expression of Vim. The positive rate of Vim and P53 is correlated with cytological differentiation of the carcinoma cells. Conclusion:Biomarkers CK7, Vim, and P53 are playing pathogenic roles, assuming as a mutual transcriptional modulator, and Vim but not P53 is likely the favorable prognostic factor, in the development of variant subtypes of endometrial carcinoma in addition to a evaluating the treatment.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2005年第1期81-84,共4页
Journal of Peking University:Health Sciences
