摘要
酶法从牛骨中制取照相明胶的新工艺与传统的碱法相比具有生产周期短、好胶率高以及产品质量相近等特点。应用差热分析研究了胶原蛋白在骨头预处理过程中的变性情况;用颗粒分布仪研究了骨素的溶胀作用;用凝胶过滤色谱技术测定了明胶分子量的分布;应用响应曲面分析优化了酶解工艺条件即1g骨素粉加1u的蛋白酶于27℃,pH7.5左右下水解6h。并对该工艺所得明胶产品的理化性能和照相性能进行了测定,结果表明该产品可用作国标中惰(Ⅰ)型照相明胶。
A new technology of preparing photographic gelatin from bovim bones by enzy-matic procedure has been studied in this paper. Compared with traditional alkalinemethod, the advantages of the new technology are shorter process period, higher yield ofhigh—quality gelatin and quality stable etc. In this paper, the denaturation of the collagenin the pretreatment of bone was studied by mean of the differential thermal analysis. Theswelling of ossein particle was determined by using an automatic particle analizer. The dis-tribution of molecular weight of gelatin was determined by gel filtration chromatography.The optimum operation parameters of enzymatic hydrolysis derived from response surface analusis (RSA) include 1 unit protease per gram dried ossein, and 6 hours of hydrolyzing time at 27℃ and pH 7.5+. And the physical, chemical and photographic properties of the gelatin product made from the new technology were determined, The result showed that it meets the official quality standards, and can be used as type(Ⅰ) inert photographic gelatin from bone.
关键词
明胶
胶原
蛋白酶
照相明胶
Gelatin
Collagen
Protease
Photographic gelatin
