摘要
以活体种子根为材料,采用放线菌酮预处理,直接获得了清晰的黄瓜前中期染色体C-分带。黄瓜前中期染色体的长度介于3～8μm,单套染色体组具有21条稳定的C-带,包括12条末端带、7条着丝点带、1条中间带和1条随体带。还探讨了影响前中期C-分带制备的关键因素,提出了黄瓜前中期染色体C-分带的实用制备方法。
Clear C-bands at prometaphase were obtained in cucumber(Cucumis sativus L.,2n=2x=14)when cycloheximide was applied to the germinating roots in vivo as pretreatment. Chromosome length at prometaphase varied from 3 to 8μm. Haploid complement had 21 distinct and stable C-bands, including 12 telomeric-, 7 centromeric-,1 intercary- and 1 satellite-bands. Some factors affecting the band formation were investigated and a practical procedure of C-banding of prometaphase chromosome was proposed.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第6期814-816,共3页
Acta Horticulturae Sinica
基金
国家高技术研究发展计划专项(2002AA241251
2002AA207012)
国家自然科学基金资助项目(30170644
30470120)
教育部跨世纪优秀人才培养计划项目
南京农业大学青年科技创新基金项目(KJ04009)
关键词
黄瓜
染色体
C-分带
前中期
Cucumber
Chromosome
C-banding
Prometaphase
