摘要
对自欧美和亚洲不同地理环境、不同科属寄主植物根瘤分离的35株菌的研究结果表明,菌株均具有典型的弗兰克氏菌特征。其中由胡颓子属植物分离的4株菌具有串珠状特异菌丝体结构。全细胞氨基均含有 meso-DAP,全细胞糖均为 D 型。均具有不同程度的固氮酶(乙炔还原)活性,并在乙炔注入后24—72小时活性倍增,同时在48—72小时活性达到最高。根据对糖和丙酮酸钠的利用,可将供试35株菌分为2群4亚群。
35 strains of Frankia were collected from root nodules of various family and genus of plant hosts in different geological environment of America,Europe and Asia.The result indicates that under pure cultivation,the strains tested showed the typical morphological characteristics of Frankia,some of them may have specialized torulose hyphe.All Frankia strains tested showed presence of the meso-DAP and whole-cell sugar pattern D.All of them showed a marked increase in nitrogenase activity from 24—72 h after the addition of acetylene,and the maximum nitrogenase activity can be achieved at 48h or 72h. Physiological result were assessed in order to compare 35 Frankia strains tested,based on the different utilization of carbohydrates at a 0.5%(W/V)concentration and organic acid salts at a 0.2% (W/V)concentration,the strains isolated from 8 geners of angiospermous plant hosts can be classified into 2 groups (including 4 subgroups).
出处
《微生物学报》
CAS
CSCD
北大核心
1993年第4期297-303,共7页
Acta Microbiologica Sinica
基金
国家自然科学基金重大项目资助
