摘要
目的利用cDNA表达阵列构建遗传性癫痫大鼠海马基因表达谱,寻找其中的差异表达基因,为从分子水平探讨癫痫的发病机理打下基础。方法采用32P-α-dATP逆转录标记探针与cDNA阵列杂交,构建P77PMC大鼠和Wistar大鼠海马基因表达谱,用图象分析仪分析两者基因表达谱差异。结果在P77PMC大鼠海马中共发现有15个差异表达基因,其中12个基因表达上调,3个基因表达下调。并用逆转录-聚合酶链反应进一步证实了结果的可靠性。结论P77PMC大鼠与正常Wistar大鼠海马中存在多个差异表达基因,这些差异表达的基因可能在癫痫的发生中具有重要作用。
Objective To find out the differentially expressed genes in the hippocampus of the rats with genetic epilepsy so as to lay a foundation for exploring the pathogenesis of epilepsy by means of cDNA array technology. Methods Gene expression patterns in the hippocampus of the genetic epilepsy prone P77PMC rats and normal Wistar rats were established using the α 32 P labeled cDNA probes hybridized with the Atlas TM Rat cDNA Expression Array, and then were analyzed by an image analysis instrument to get the differentially expressed genes. Results Fifteen genes were found having differential expression patterns in hippocampus between the P77PMC rats and the Wistar rats, while there may be many other differentially expressed genes left undiscovered due to having no appropriate image analysis software. And among the fifteen genes, the expression levels of twelve genes in the P77PMC rats were higher than those in the Wistar rats, while the expression levels of the other three genes were lower. The results of reverse transcription polymerase chain reaction(RT PCR) have demonstrated the reliability of cDNA arrays method. Conclusion cDNA array is a powerful tool for identifying differential expression genes of epilepsy on large scales. There are several differentially expressed genes in hippocampus of the P77PMC rats and the Wistar rats. All these identified genes could play potentially important roles in the pathogenesis of epilepsy.
出处
《中华医学遗传学杂志》
CAS
CSCD
2004年第6期619-621,共3页
Chinese Journal of Medical Genetics
基金
教育部高等学校骨干教师资助计划项目(2000-65)~~
