摘要
对γ 射线诱导中国仓鼠成纤维(CHL)细胞基因组不稳定性进行了研究。利用 CB 法微核检测技术对一次照射、二次照射的 CHL 细胞进行微核测定,观察 DNA 损伤程度的变化情况。结果显示,微核发生率与细胞受照剂量呈相关关系,并随培养时间延长呈下降趋势。细胞再次受照后,其微核发生率与一次受照剂量呈相关关系。表明微核发生率与染色体损伤程度存在剂量相关性,微核检测技术可以用于生物效应表现为染色体损伤的辐射诱导基因组不稳定性的研究。
To analyze the genomic instability induced by γ-rays, cytokinesis-block MN assay was employed to investigate the gene damage of cells and their progeny cells exposed to radiation. The micronucleus frequency were related to the irradiation dose and decreased as the culture time lengthened. There was a correlation between the micronucleus frequency and the initial dose after the second irradiation. The micronucleus frequency may be causally related to radiation-induced genomic instability which is expressed as chromosomal damage leading to micronuclei.
出处
《辐射研究与辐射工艺学报》
EI
CAS
CSCD
北大核心
2005年第1期45-48,共4页
Journal of Radiation Research and Radiation Processing
