摘要
目的 :应用聚合酶链反应 (PCR)技术检测早、中期自然流产患者胚胎及胎盘等组织中的微小病毒B19,对比分析早期流产的原因。方法 :收集早期自然流产患者的胚胎绒毛组织和母亲血清 ,孕中期自然流产胎儿的胎盘、胎儿组织和母亲血清 ,足月分娩者的胎盘组织及母亲血清 ,应用PCR检测微小病毒B19DNA ,同时检测母血清抗体。结果 :6 1例早期流产胚胎绒毛中 ,3例 (5 % )B19病毒阳性 ;4 5例孕中期胚胎组织中 ,5例 (11% )检测出B19病毒 ;5 0例足月分娩胎盘组织中未检测出B19病毒。早期流产患者血清中特异性抗体IgG检出 31%、IgM阴性 ;检出孕中期母血清B19病毒特异性抗体IgG占 35 %、IgM占 11%。结论 :B19病毒是导致早期流产的原因之一 。
Objective:To detect the infective rate of parvovirus B19 in the placenta and fetal tissues among the first-and second-trimester spontaneous miscarriage by PCR techniques and to analysis the reason of abortion.Methods:Placenta and/or fetal tissues from the first-,second-trimester fetal losses,and placenta from full-term normal pregnancies were examined for presence of B19 DNA by PCR in a prospectively study from 2000 to 2002.B19 immunoglobulin IgM and IgG were also detected in maternal serum in these three stages.Results:The positive B19 DNA was detected in 3 of 51 (3%) placenta tissues from the first-trimester fetal losses,5 of 45 (11%) from the second-trimester fetal losses,and none from full-term pregnancies.The positive B19 immunoglobulin IgG and IgM were 31% and 35%,0 and 11% in the maternal serum of the first-and the second-trimester fetal losses,respectively.Conclusions:Human parvovirus B19 is one of the reasons that caused the first-trimester abortion.Detection B19 DNA by PCR is an important tool to diagnose the parvovirus B19 infection in the first-trimester spontaneous miscarriage.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2005年第1期51-52,共2页
Journal of Practical Obstetrics and Gynecology
