摘要
从胎肝和肝癌组织中纯化的 GGTP,经4%—30% PAGE 鉴定氛明分成二部分,其中第一部分活力最强,经亲和层析柱吸附后,免疫动物获纯的抗血清.该部分 GGTP 经10% PAGE 显示一条带,其分子量为92kD,pI 值为4.2.但经 SDS-PAGE 则显示二条蛋白带,其分子量分别为64kD 和27kD.酶免疫抑制试验表明该抗血清对 GGTP Ⅱ,Ⅱ’具有抑制作用,双扩散免疫试验产生单一沉淀线,并互相融合在一起.说明二种组织来源的 GGTP 的免疫学特性相一致.
To elucitate characteristics of the novel GGTP specific to liver cancer cells,GGTPsfrom human fetal liver and hepatoma tissues were purified by Triton X-100 and deoxy-cholate extraction,cold acetone precipitation,glutathione treatment and bromelain digestionfollowed by Sephadex G-150 gel filtration and DEAE-cellulose DE-52 chromatography. The enzymes purified from two sources displayed a pI of 4.2 and an apparent M_r ofabout 92kD,as judged by polyacrylamide gel electrophoresis,which consists of two non-identical glucopeptides with M_r of 64 kD and 27 kD,respectively.The purified GGTPs were used to produce antibodies in rabbits.The antisera withtiters of 1∶16 were observed to be capable of being absorbed by the constituents of thenovel GGTP,Ⅱ and Ⅱ′,in sera of patients with hepatoma.Furthermore,they could formin the double diffusion test a smoothly fused precipitating line with two kinds of purifiedantigens.These results show that GGTPs from human hepatoma and fetal liver tissues are ofimmunological identity.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1993年第1期32-37,共6页
Progress In Biochemistry and Biophysics
关键词
胎肝
肝癌组织
谷氨酰转肽酶
Gamma-Glutamyltranspeptidase
fetal liver hipatoma tissue
