摘要
目的:探讨外周血T淋巴细胞功能相关性标志物在传染性单核细胞增多症(IM)患儿急性期、恢复期中的变化规律。方法:采用流式细胞术(FCM)检测21例急性期IM患儿和其中8例恢复期随访患儿的外周血T淋巴细胞功能相关性标志物、CD3+、CD3+CD4+、CD3+CD8+、CD3+CD4+CD8+、CD4+CD25、CD8+CD25+、CD8+CD28+、CD4+CD69+、CD4+CD45RA+、CD4+CD45RO+并与10名正常对照组比较。结果:IM患儿CD3+细胞比例,CD8+CD28+细胞比例与对照组比较无统计学差异,CD4+、CD4/CD8比值,CD4+CD25+,CD4+CD69+,CD4+CD45RA+,CD4+CD45RO+比例均明显降低。CD8+T淋巴细胞,CD8+CD25+T淋巴细胞比例明显升高。随访病例中,CD3+CD8+T细胞比例及CD4+CD69+T细胞比例在疾病急性期与恢复期无明显差异,其他指标则差异有显著性;将恢复组与正常对照组比较,CD4/CD8比值差异仍有显著性,恢复期与正常对照间其他标志物已基本无显著差异。结论:IM患儿存在细胞与体液免疫功能紊乱,T淋巴细胞在整个疾病过程中起主要作用,对IM患儿应长期密切随访。
Objective To study the changes and significance of the T lympolocyte function related markers in the peripheral blood of the children with infectious mononucleosis (IM). Methods The T cell function related markers such as CD3, CD4/CD8, CD25, CD28, CD69, CD45RA and CD45RO were analyzed by flow cytometry in 21 children at the acute stage of IM and 8 clildren recovering from IM in comparison with the ratios of 10 healthy controls. Results There was no significant difference between the IM patients and the normal controls in the ratios of CD3+ and CD8+CD28+ T cells. Compared with the following results in controls, CD4+, CD4/CD8 ratio, activated T cell markers like CD25 and CD69, CD4+/CD45RA+ and CD4+/CD45RO+ T cells were significantly reduced, CD8+ and CD8+/CD25+ T cells were significantly increased in the IM patients. In the childern recovering from IM, all the markers except CD3+/CD8+ and CD4+/CD69+ were significantly different from the ones at the acute stage. There was no obvious difference in all the marks except CD4/CD8 ratio between the children recovering from IM and the nomal controls. Conclusions There lies the cellular immune dysfunctions in the children with infectious mononucleosis. T cells play an important role in the disease course. The patients with IM are subjected to the close follow-up for the development of the malignancy.
出处
《诊断学理论与实践》
2004年第6期427-430,共4页
Journal of Diagnostics Concepts & Practice
