摘要
目的 研究眼睑鳞状细胞癌 (SCC)血管内皮生长因子 (VEGF)表达水平。方法 8例眼睑SCC患者血清标本和 8例非肿瘤患者血清标本 ,用酶联免疫吸附测定 (ELISA)方法测定血清中VEGF浓度 ;2 0例眼睑SCC病理标本和 2 0例正常眼睑组织标本 ,用免疫组化方法 ,检测标本中VEGF表达水平。结果 实验组血清中VEGF平均浓度为 (333 88± 73 32 )pg/ml,对照组血清中VEGF平均浓度为 (10 9 75± 2 6 4 1) pg/ml,差异有显著性 (P <0 0 1) ;眼睑SCC标本VEGF平均染色分数为 2 37± 0 14 ,对照组为 0 5 4± 0 16 ,存在显著性差异 (P <0 0 1)。结论 VEGF在眼睑SCC患者血清及其瘤体中的表达显著升高 ,VEGF可能通过促进新生血管生成在眼睑SCC发生发展过程中发挥重要的调控作用。
Objective To detect the expression of vascular endothelial growth factor (VEGF)in eyelid SCC. Methods 8 consecutive patients who had undergone curative resection of primary eyelid SCC and 8 voluntary control subjects were obtained.Serum levels of VEGF were determined by quantitative sandwich enzyme immunoassay technique(ELISA).20 tumor samples of eyelid SCC and 20 matched tissues of healthy subjects undergoing blepharoplasty were collected.Staining for VEGF were analyzed immunohistochemically. Results The mean concentrations of serum VEGF were (333 88±73 32)pg/ml in study group and (109 75±26 41)pg/ml in normal controls.VEGF levels of serum were significantly increased in study group as compared with those in control subjects( P <0 01).The average scores of VEGF staining were 2 37±0 14 in tumor specimens and 0 54±0 16 in normal tissues.There is a significant difference between two groups( P <0 01). Conclusions Eyelid SCC was associated with significantly elevated expression of VEGF.VEGF may play a crucial role in the control of angiogenesis in eyelid SCC which may be responsible for rapid growth、local invasion and distant metastasis of evelid SCC.
出处
《中国实用眼科杂志》
CSCD
北大核心
2005年第1期28-31,共4页
Chinese Journal of Practical Ophthalmology
