摘要
目的 :研究自拟温下方逆转MCF 7 ADM细胞耐药、诱导凋亡作用。方法 :MTT法研究温下方含药血清对MCF 7 ADM耐药逆转作用 ,流式细胞术测定细胞内药物浓度及耐药相关蛋白P gp、凋亡调控蛋白p5 3、bcl 2的表达 ,激光共聚焦显微镜技术检测细胞内钙浓度及线粒体膜电位。结果 :温下方含药血清可提高MCF 7 ADM对化疗药的敏感性 ,增加胞内化疗药物浓度 ,使P gp表达下降 ,p5 3表达增高 ,胞内钙浓度及线粒体膜电位均降低。结论 :温下方可部分逆转MCF 7 ADM耐药 ,其逆转机制可能与诱导耐药细胞凋亡密切相关。
Objective:To study the effects of compound prescription on reversing drug resistance and apoptosis of MCF 7/ADM.Methods:Using tetrazolium dye assay to study the reversal effects of serum containing compound prescription on MCF 7/ADM.Intracellular ADM concentration,MDR related protein P gp,apoptosis adjusted protein p53 and bcl 2 were detected by flow cytometry(FCM); intracellular Ca 2+ concentration and mitochondrion membrane potential were observed by Laser Scanning Confoca Microscopy (LSCM).Results:Serum containing compound prescription could enhance sensitivity of MCF 7/ADM on ADM and intracellular ADM concentration and expression of p53,reduce expression of P gp and intracellular Ca 2+ concentration and mitochondrion membrane potential.Conclusion: Serum containing compound prescription could reverse multidrug resistance in MCF 7/ADM,which may be closely related to the mechanism of its inducing apoptosis of MCF 7/ADM.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2005年第1期19-21,24,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
山东省优秀中青年科学家科研奖励基金课题 (0 1BS5 5 )
关键词
温下方
逆转耐药
诱导凋亡
乳腺癌耐药细胞株
compound prescription
reversing drug resistance
inducing cell apoptosis
MCF-7/ADM cell line
