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应用兔抗体放免药盒测定兔血清性激素含量的方法探讨

INVESTIGATION OF THE METHOD TO DETECT THE CONTENT OF THE SEX HORMONE USING RIA KIT WITH RABBIT-ANTIBODY
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摘要 应用兔抗体的FSH,LH,E_2,P4种碘标放免药盒,经过添加试剂,改良反应条件和改换分离剂,不经提取待测成份,就可正确满意地测定兔血清FSH,LH,E_2和P的含量。作者建立的增二抗-NRS-PEG改良方法可作为兔抗体的放免药盒(不经提取法)检验兔血清标本的通用方法。 Using FSH,E2,LH and P RIA kit of rabbit-antibody,we don′t extract unknown different compontent.With these samples,the content of FSH,LH,E2 and P of rabbit serum were detected,while changing the separational agent and adding some agents,as well as mordifying the reaction condition.It was proveded that not all the RIA kit of r-Ab can perform in quantity detection only with the changing of separational agent.For this reason,we established“increase double Ab-NRS-PGE”mordified method.It can do the job and used as general method。
出处 《山东医科大学学报》 1993年第1期13-16,共4页 Acta Academiae Medicinae Shandong
关键词 抗体 性激素类 放射免疫测定 Antibodies Sex hormones Radioimmunoassay Rabbits
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