摘要
在长日照条件下,10-7mol/L的二苯脲对紫萍半叶状体就有保绿的作用,10-5～2×10-4mol/L的二苯脲还可以明显地促进叶绿素的合成和干重的增加,提高丝氨酸乙醛酸转氨酶的活性,延缓半叶状体的衰老.实验结果表明,二苯脲本身具有细胞分裂素的活性,能代替内源细胞分裂素起到抗衰老的作用;二苯脲同时也能推迟半叶状体中内源细胞分裂素含量的降低.另外,在培养液中加入外源L-丝氨酸,可以抗拮二苯脲对紫萍半叶状体衰老的延缓作用,说明二苯脲抗衰老的机制类似于其它细胞分裂素.
DPU at a concentration of as low as 10^-7 mol/L retained the chlorophyll of Spirodela half-fronds under long-day conditions. From 10^-5 ～2×10^-4 mol/L it prompted the synthesis of chlorophlly and the serine:glyoxylate aminotransferase (SGAT) activity, increased dry weight, and retarded the senescence of half-fronds. It was demonstrated that DPU itself acted as a kind of cytokinin in delaying senescence. However, it also postponed the decline of endogenous cytokinins of half-fronds. L-serine added into the medium (10^-3 mol/L) antagonized the effect of DPU, which shows the mechanism of DPU in delaying senescence is most likely similar to other cytokinins.$$$$
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第4期90-94,共5页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家自然科学基金资助项目(39970077)
关键词
二苯脲
紫萍
半叶状体
延缓衰老
作用机制
diphenylurea
Spirodela polyrrhiza
half-fronds
delaying senescence
regulation mechanism
