摘要
以感染性克隆SHIV-KB9cDNA为主要骨架,将其env基因区域的gp120和部分gp41(从N末端的50到650个氨基酸)的1.7kb片段,用来源于4个不同中国HIV-1B′/C毒株env的相应区域进行替换,构建了50个全基因SHIVcDNA克隆,大规模体外活性筛选实验,获得了能够在人的PBMC中进行生产性复制的SHIV-XJ02170cDNA克隆.
full-genome Chinese SHIV clones were constructed by replacing the gp120 and partial gp41 (Covering N-terminal 50 to 650 amino acids) fragment of infectious SHIV-KB9 clone, with the corresponding region of four different Chinese HIV-1 B′/C isolates. The infectivity of all SHIV clones was tested individually in human PBMC. Two SHIV-XJ02170 in fectious clones were screened from the 50 clones and it could replicate in human PBMC.$$$$
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第4期84-89,共6页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
美国NIH在中国的CIPRA基金资助项目(U19AI051915)
