摘要
用小鼠泡球蚴(MAH)和沙鼠泡球蚴(GAH)抗原ELISA对-20℃贮存3.5～6年的泡球蚴患者抗体作了检测。结果两种抗原敏感性各为98.1％和100％,与3.5～6年前的敏感性100％无统计学差异。用GAH抗原检测,50％病例抗体滴度下降,但38.5％病例抗体滴度增加,还有11.5％抗体滴度相同。在滴度下降病例中,76.9％下降1～2个滴度。贮存3.5～6年的抗体,其滴度在1:1600以上者仍占71.2％,且几何均数为原来用细粒棘球蚴液的1.1746倍,说明沙鼠泡球蚴抗原是检测泡蚴病的最适抗原。
The antibody in patients with multilocular echinococosis stored at-20℃ for 3.5—6 years was determined by ELISA with antigen from mice alveolar hydatid (MAH)and gerbil nlveolar hydatid(GAH). The sensitivities of MAH and GAH antigen were 98.1% and 100 %, respectively. The difference of the sensitivities between the before and after 3.5—6 years was not significant (t=1.005). It was suggested that the activity of antibody stored at-20℃ was not decrased. Compared with the results tested with antigen GAH 3.5— 6 years ago the antibody titer decreased in 50%cases, increased in 38.5% and unchanged in 11.5%. The antibody titer of 76.9% cases with titer decreased was decreased by 1—2 titers and the titers in 71.2% of cases was higher than 1:1600. The geometric mean titers with GAH antigen was 1.17 times of those with Eg HF antigen 3.5—6 years ago. It was suggested that gerbil alveolar hydatid wat the most appropriate antigen in determining alveolar hydatidosis.
出处
《宁夏医学杂志》
CAS
1993年第4期201-203,共3页
Ningxia Medical Journal
关键词
泡球蚴病
血清
低温保存
抗体效价
Multtlocular echinococ cosis
Alveolar hydatid
Antibody
