摘要
用酒石酸钾─甘油梯度离心纯化的RHDV抗原免疫BALB／C小鼠，取脾细胞与SP2／0融合．获得4株稳定分泌RHDVMcAb的杂交瘤细胞株（1F7，1H4，2A9，2D6）。经检测，所分泌的抗体亚类分别为IgG2a（1F，1H4，2D6）和IgG1（2A9）。应用双抗体夹心ELISA法检测70份标本，并与直接免疫荧光法和HA法比较，取得较好的实验结果。
Four strains of hybridoma cells secreting speafic antibodies to RHDV were obtained by cellfusion technique. The RHDV used for immugon was purified by potassium Tartrafe-Glyceraldensity gradient Centrifugation. It was confirmed by western blot that the McAbs react with60KD, 55KD, 38KD and 20KD of RHDV protein. Double-McAb sandwich ELISA for the defection of RHDV antigen was established and analysis of sensitivity and specificity on test systemwas also performed in our laboratory.
出处
《中国实验动物学杂志》
1998年第4期216-220,共5页
Chinese Journal of Laboratory Animal Science
