摘要
目的 观察气道内干扰素 -γ(IFN -γ)基因转染对哮喘小鼠支气管肺泡灌洗液 (BALF)嗜酸性粒细胞、中性粒细胞和淋巴细胞的影响 .方法 C5 7BL/ 6小鼠 4 0只 ,随机分为 4组 ,每组 10只 ,分别为正常对照组、哮喘组、模型空质粒干预组 (空质粒组 )和模型干扰素质粒干预组 (干扰素组 ) .卵白蛋白 (OVA)抗原溶液腹腔注射致敏 ,滴鼻激发造模 .对照组用生理盐水代替OVA ;空质粒组和干扰素组分别经鼻滴入空质粒或重组干扰素质粒 .观察各组实验小鼠的哮喘症状以及BALF中IFN -γ水平和各类炎症细胞的变化 .结果 哮喘组BALF中IFN -γ水平比正常对照组显著降低 ;哮喘小鼠干扰素质粒气道内转导后哮喘症状明显减轻 ,BALF中IFN -γ水平显著升高 ,同时嗜酸性粒细胞、中性粒细胞和淋巴细胞显著减少 .结论 气道内转染干扰素质粒能有效改善哮喘小鼠的症状和气道炎症 .
Objective To study the effect of IFN-γ plasmid ge ne transferring to tracheal on numbers of eosiphils,neutrophils and lymphocytes in bronchoalveolar lavage fluid(BALF) of mice asthma model. Methods Forty C57BL/ 6 mice were randomly divided into four groups: the control group, the model grou p, the plasmid group and the interferon group(IFN group); 10 mice in each grou p. Except the control group, other groups were sensitized with OVA by a combinat ion of intraperitoneal injection and repeated intranasal challenges to establish the mice asthma model. In the control group, normal saline was instead. In the plasmid and IFN group, the plasmid and IFN-γplasmid were given to asthma mice b y intranasal drip.Asmatic symptom of the mice was observed in each group.The lev el of IFN-γ,numbers of eosiphils,neutrophils and lymphocytes in BALF of the mi ce was examined. Results The IFN-γin BALF of the model group was lower than the control group. Asmatic symptom was better in IFN group than in the model group and the plasmid group.The IFN-γ in BALF of IFN group was higher than the control group, the mo del group, the plasmid group. But the change of eosiphils,neutrophils and lymph ocytes in BALF was contrast with the IFN-γ. Conclusions These f indings suggest that transtrancheal IFN-γ gene transfer is effective in improvi ng symptom and airways inflammation of asthma mice, which provides a basis for d eveloping a novel therapeutic approach.
出处
《现代临床医学生物工程学杂志》
2004年第5期375-377,共3页
Journal of Modern Clinical Medical Bioengineering
基金
广东省自然科学基金重点攻关项目 (A0 0 0 0 990 36)
