摘要
我们检测了IL-3,IL-6和IL-4单独和联合G-CSF和GM-CSF对小鼠异基因骨髓移植后造血重建的影响.IL-3+GM-CSF是增加移植后6d中性粒细胞绝对计数最有效的联合,显示显著的协同作用.当单独给予时,IL-6和G-CSF分别显著增加移植后6d血小板计数,而在联合给予中,只有IL-3+G-CSF显著增加移植后6d的血小板计数.IL-4对移植后7d中性粒细胞恢复有显著的抑制作用.对由IL-3和G-CSF加速的中性粒细胞恢复亦有显著的抑制作用.
Abstract We investigated the effects of IL-3, IL-6 and IL-4, alone and in combination with G-CSF and GM-CSF on hematopoietic reconstitution following murine allogenic bone marrow transplantation. The most effective combination for increasing the circulating absolute neutrophil count (ANC) above the control value at day 6 posttransplant was the combination of IL-3 and GM-CSF and showed a significant synergistic effect. When used alone, both IL-6 and G-CSF raised platelet count at day 6 after transplantation (312×10^(9)/L, P=0.02 and 309×10^(9)/L, P=0.01 respectively) compared to control mice(216×109/L). In combination, only IL-3 with G-CSF significantly increased the day 6 platelet count (328×10^(9)/L). We found that neutrophil recovery at day 7 after transplantation was significantly suppressed by IL-4 in a dose-dependent fashion and that the recovery of nentrophils accelerated by IL-3, and G-CSF was also significantly suppressed by IL-4.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
1995年第2期135-138,共4页
Chinese Journal of Cancer Biotherapy
