摘要
从裸燕麦麸皮中提取β 葡聚糖,用硫酸铵沉淀法对粗提物进行初步纯化得到组分POG,利用离子交换柱色谱和SepharoseCL-4B凝胶柱色谱对POG进一步纯化得到组分POG11和POG12,用气相色谱测定了它们的单糖组成,并经荧光吸收表明组分POG11为β 葡聚糖,用酶法测定其β 葡聚糖含量为98.6%,高效液相色谱显示该组分相对分子质量为2.62×106,红外光谱和13C核磁共振表明POG11是混合键(1→3)(1→4)-β-D-葡聚糖.
β-Glucan was extracted and purified from naked oat bran by adding (NH_4)_2SO_4 and using ion-exchange as well as gel-filitration. POG11 was mainly composed of glucose. The purity was analyzed by fluorescence detection and β-glucan's content was 98.6% quantified by enzyme. Its molecular weight detectded by HPLC was 2.62×10~6 . The structure of the isolated β-Glucan showed that the glucose units were joined with 1,3 and 1,4-lingage by Infrared spectral and ()^(13)C-NMR detection.
