摘要
AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori(H pylori) and to study the immunogenicity of BabA.METHODS: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore,BabA immunogenicity was studied by animal test.RESULTS: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank.The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of Hpyloriinfection.
AIM:To construct a recombinant strain which expresses BabA of Helicobacter pylori(Hpylon)and to study the immunogenicity of BabA. METHODS:BabA_2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b(+)and expressed in the BL21(DE3)E.coli strain.Furthermore, BabA immunogenicity was studied by animal test. RESULTS:DNA sequence analysis showed the sequence of BabA_2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein.The serum from Hpyloriinfected patients and Balb/c rniced immunized with BabA itself could recognize rBabA. CONCLUSION:BabA recombinant protein may be an potential vaccine for control and treatment of Hpyloriinfection.
基金
Supported by the National Natural Science Foundation of China,No.30270078
