摘要
目的 研究紫外线照射减活日本血吸虫尾蚴疫苗免疫小鼠后肺部诱导型一氧化氮合酶(iNOS)的动态表达及其与保护性免疫的关系。 方法 用紫外线照射减活尾蚴疫苗免疫小鼠,30 d后用尾蚴攻击感染。于感染后第4 d、9 d取免疫小鼠和未免疫小鼠肺脏,用RT-PCR半定量的方法检测各组小鼠肺部iNOS的转录水平;用免疫组化方法确定iNOS在肺部的蛋白表达定位。 结果 正常小鼠肺部无iNOS转录和蛋白表达。免疫小鼠和未免疫小鼠感染血吸虫尾蚴后第4 d肺部iNOS有较高水平的转录表达。感染后第9 d,免疫组小鼠iNOS的表达较第4 d下降,而未免疫组小鼠肺部iNOS的表达消失。免疫组化结果表明,免疫组小鼠肺部炎症病灶细胞中存在iNOS的表达。 结论 移行到肺部的血吸虫童虫能诱导肺部表达iNOS,紫外线减活尾蚴疫苗能促使小鼠肺组织较长时间表达iNOS,iNOS在肺部的持续表达可能与该疫苗使宿主产生的免疫保护力有关。
Objective To study the levels of iNOS expression in lungs of unvaccinated mice and mice vaccinated with ultraviolet attenuated Schisto-soma japonicum cercariae and the relationship between iNOS expression and its protective mechanism in mice. Methods Mice were immunized with ultraviolet attenuated cercariae, 30 days post immunization, total RNA was extracted from the lungs of mice after a challenging infection and iNOS mRNA levels between vaccinated and unvaccinated groups at day 0, day 4, day 9 post infection were compared; Immunohistochemical test was performed to detect the distribution of iNOS in lungs. Results For both vaccinated and unvaccinated mice, there was no expression of iNOS in the lungs before challenging infection, iNOS expression increased significantly at day 4 p. t; At day 9, the expression of iNOS decreased for vaccinated mice, while there was no expression for unvaccinated mice. Result of immunohistochemical test showed that iNOS was mainly located in the inflammatory foci in the lung. Conclusion Schistosomula migrated to the lungs can induce iNOS expression in the organ, ultraviolet attenuated cercariae can prolong iNOS expression in lungs after challenging infection, it is possible that longer expression of iNOS is involved in the protective immunity induced by ultraviolet attenuated cercariae.
出处
《中国寄生虫病防治杂志》
CSCD
2004年第4期226-228,共3页
Chinese Journal of Parasitic Disease Control
