摘要
目的 测定倍他米松尿素霜中倍他米松的含量。方法 采用HPLCVP -ODS色谱柱 ,以甲醇 -水 (6 5∶35 )为流动相 ,检测波长 2 4 0nm。结果 所建方法能很好分离倍他米松和其他物质 ,在浓度 2 .6～ 10 0 .8μg·ml-1范围内 ,与峰面积呈良好的线性关系 (r=0 .99998) ;回收率为 99.4 6 % ,RSD =0 .6 6 % (n =9)。结论 所用方法专属性强 ,准确可靠。
OBJECTIVE To estabtish an HPLC method to control the quality of Betamethasone-urea cream, HPLC method was established to determine betamethasone. METHODS The chromatographic system consisted of VP-ODS column and methanol-water(65∶35) as the mobile phase with a detection wavelength at 240 nm. RESULTS Betamethasone could islated from other material by HPLC. The linear range of betamethasone was 2.6-100.8 μg·ml -1 (r=0.9999). The recovery rate was 99.46% with RSD of 0.66%(n=9). CONCLUSION This method is exclusive , accurate and simple for contents determination of Betamethasone-urea cream.
出处
《华西药学杂志》
CAS
CSCD
2004年第6期458-460,共3页
West China Journal of Pharmaceutical Sciences
