摘要
本文在自制8株神经元特异性烯醇化酶单抗(NSE-McAb)的基础上,对4株McAb分别标记辣根过氧化物酶(HRP),经多次方阵排列滴定,建立了NSE-McAb双位点夹心化学发光酶免疫分析法(CLEIA)。通过对23例SCLC,32例NSCLC,22例BPD和正常对照血清中NSE检测表明,对SCLC诊断的阳性率为78.3％,特异性为93.0％,对SCLC的临床分期和判断预后也有一定的价值。
We established two-site sandwich chemiiuminescence enzyme immunoassay (CLEIA) and preformed multiple checkboard titration, using HRP separatelly labeling 4 McAbs which purifice 8 McAbs by our laboratory. We measured the serum NSE levels of 23 patients with SCLC. 32 patients with non-small cell lung cancer (NSCLC).22 patients with benin pulmonary disease(BPD) and normal controls(NC). The positive rate of detectable was 78. 3% and specific was 93. 0% with SCLC. Two-site sandwich CLEIA may be valuable for clinical staging and evaluating prognosis in patients with SCLC.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1993年第3期194-196,202,共4页
Immunological Journal
关键词
单克隆抗体
免疫酶技术
小细胞肺癌
Neuron specific enolase, Monoclonal antibody. Two-site sandwich chemiluminescence en- zyme immunoassay,Small cell lung cancer
