摘要
本文利用双色荧光试剂、流式细胞术证明了新鲜分离的儿童扁桃体T细胞中含有少量的CD4^+CD8^+细胞。CD4^+CD8^+的复合表达似乎与T细胞活化有关,其主要表达在大的淋巴母细胞上。用PHA刺激T细胞5天后,产生的CD4^+CD8^+细胞数可达38.33％;与此同时,细胞上CD8抗原的密度明显增高。另外,PHA活化的CD4^+CD8^+细胞能高水平表达CD25、CD38及HLA-DR抗原,但缺乏CD1抗原的表达。我们的实验结果提示:人扁桃体T细胞活化产生的CD4^+CD8^+细胞亚群不同于CD4^+CD8^+的所谓双阳性胸腺细胞,由于CD4和CD8分子直接与MHC抗原相互作用,这些分子的复合表达可能在免疫调节中起重要作用。
Using two-color fluorescence flow cytometry, we were able to detect the presence of small numbers of CD4+CD8'l'cells(3. 29±1. 14%)in freshly isolated T cell populations derived from human tonsil. Coexpression of CD4 and CDS was predominantly found on large blastlike cells and appeared to be related to activation. Stimulation of T cells with PHA for 5 days resulted in the significant increase of CD4','CD8+ cells. Coexpression was accompanied by significant increase of CDS antigenic densities. The PHA-activated CD4+ CDS'1' cells expressed high levels of activation antigens CD25.CD38 and HLA-DR,but lacked GDI antigen. Our data suggested that tonsillar T cell activation results in the generation of CD4+CD8+ cells which is distinct from thymic cells. Because CD4 and CDS molecules may interact directly with MHC antigens. Coexpression of these molecules may have an important role in immune regulation.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1993年第3期160-163,共4页
Immunological Journal
关键词
扁桃体
流式细胞术
T细胞
抗原
Tonsil,Flow cytometry.Two-color immunofluoresence
