摘要
作者从水牛鼻表皮中分离桥粒,提取桥粒中的Desmoplakin I(DPI)。用DPI作免疫原,免疫BALB/C小鼠,建立了一株分泌抗DPI单抗的细胞AD-1。免疫组化染色证实,该单抗与上皮组织呈阳性反应,与非上皮性组织呈阴性反应。提示该单抗可作为区分上皮性肿瘤与非上皮性肿瘤的免疫组化探针。
Desmosomes were isolated from cow nose epidermis. Desmoplakin KDPDwas purified from desmosomes by preparative electrophoresis. The spleen cells of BALB/C mice immunized with DP I were fused with myeloma cells line SP2/0. Screening for antibody activity in cultured supernatants was carried out by ELISA and immunohistochemical staining on frozen sections of human skin. After positive hybridomas were subcloned three time by limiting dilution, a mouse hybridoma, AD-1, producing monoclonal antibody (McAb) to DP I was established. The tissue specificity of the McAb was tested on normal tissue frozen sections with immunohistochemical technique. All the epithelia tested were stained positively with AD-1 McAb, whereas nonepithelia tested showed negative staining. It is suggested that AD-1 McAb may be used as immunohistochemical probe for differentiation of epithelial tumors and nonepithelial tumors.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1993年第1期35-37,共3页
Immunological Journal
基金
国家教委博士点基金资助项目
关键词
单克隆抗体
制备
桥粒
肿瘤
Preparation, Desmoplakin I. Monoclonal antibody
