摘要
为探讨脊髓Ｐ物质（ＳＰ）在自身免疫性疾病发病中的作用，本实验以豚鼠（ＧＰ）全脊髓匀浆（ｓｐｉｎａｌｃｏｒｄｈｏｍｏｇｅｎａｔｅ，ＳＣＨ）为抗原建立了实验性变态反应性脑脊髓炎（ｅｘｐｅｒｉｍｅｎｔａｌａｌｌｅｒｇｉｃｅｎｃｅｐｈａｌｏｍｙｅｌｉｔｉｓ，ＥＡＥ）模型。从皮下注射辣椒素（ｃａｐｓａｉｃｉｎ，Ｃａｐ）以耗竭脊髓ＳＰ，观察其对ＥＡＥ发病及免疫功能的影响。结果表明：单纯注射辣椒素组豚鼠不发生ＥＡＥ，但皮下注射辣椒素１４ｄ后，再以ＳＣＨ免疫动物则发病率增高，病情加重，潜伏期缩短，并伴有细胞及体液免疫功能亢进，表现在淋巴细胞转化水平及血清抗ＳＣＨ抗体滴度增高；在ＥＡＥ发病高峰期脊髓后角及后根神经节ＳＰ含量明显增高，提示脊髓后角及初级感觉神经元中ＳＰ参与ＥＡＥ发病过程。这部分ＳＰ具有抑制免疫的功能，在ＥＡＥ发病高峰期脊髓后角及后根神经节ＳＰ含量增高可反馈性地抑制免疫反应使之不致过强，故具有保护性意义。
The experimental allergic encephalomyelitis(EAE)was induced by immunization with guinea pig spinal cord homogenate(SCH)in complete Freund's adjuvant(CFA)in guinea pig.In order to investigate the effect of depletion of substance P on clinical severity and immunological pathogenesis of EAE,guinea pigs were administrated with capsaicin subcutaneously prior immunization,Capsaicin pretreatment could increase the incidence of EAE,aggravate clinical severity and shorten the latency of disease significantly,accompanied by an enhancement of cellular and humoral immune responses,The substance P content of the spinal dorsal horn(L3-5)and dorsal root ganglia(L3-5)decrease markedly in the capsaicin-pretreated group at the peak of immune response, but increase at the same area in the EAE animal without capsaicin-pretreated compared to that of group inoculated only with CFA. These results suggest that substance P in the spinal dorsal horn and dorsal root ganglia could suppress immunity, while the increase of substance P contents at the peak of immune response may give a negative feedback to immunity via unknown pathway.Thus,substance P might play an important role in the pathogenesis of EAE.
出处
《中国神经免疫学和神经病学杂志》
CAS
1995年第1期17-21,共5页
Chinese Journal of Neuroimmunology and Neurology
基金
国家自然科学基金
